L-Carnitine Improves Cytoprotection during Cryopreservation: A case study on Nili-Ravi Buffalo Sperm

Authors

  • Saima Qadeer Department of Zoology, Division of Science and Technology, University of Education, Lahore-54000, Pakistan
  • Rabea Ejaz Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar-25000, Pakistan
  • Asma Ul Husna Department of Zoology, University of Sialkot, Sialkot-51310, Pakistan
  • Asima Azam Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar-25000, Pakistan
  • Syeda Laila Rubab Department of Chemistry, Division of Science and Technology, University of Education, Lahore-54000, Pakistan
  • Ghulam Nabi School of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei, China
  • Sana Ullah University of Education Lahore
  • Shamim Akhter Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-46300, Pakistan

Keywords:

buffalo, sperm, cryopreservation, extender, L-Carnitine, artificial insemination

Abstract

The current study was aimed to evaluate the antioxidative effect of L-Carnitine at post thawing following cryopreservation of Nili-Ravi buffalo sperm. For the purpose, semen from three buffalo bulls were collected for 3 weeks using artificial vagina (N=18; replicates). The qualified ejaculates were diluted employing tris-citric acid extender i.e., control did not receive any L-Carnitine and experimental groups having 0.5, 1.0, and 1.5 ng/mL of L-carnitine at 37°C with approximately 50 × 106 sperm/mL. The semen wascooled at 4°C and then equilibrated (4 hours), filled in straws (0.5 mL) at4°C, placed on LN2 vapours for 10 min and kept into an LN2 container. Thethawed semen was evaluated for post-thaw quality. The integrity of thesperm plasma membrane and motility (P˂0.05) was highest in theextenders having 1.0 ng/mL of L-carnitine as compared to the control(received no L-Carnitine). However, sperm chromatin integrity and viability(live sperm with intact acrosome) remained similar. It was concluded thatsupplementing 1.0 ng/mL L-Carnitine of extender can improve the post-thaw quality of cryopreserved sperm. Based on the results of the currentexperiments it is recommended to include L-carnitine extender to improvepost-thaw quality of buffalo sperm in terms of its motility and integrity ofits plasma membrane.

Published

2021-09-11

How to Cite

Qadeer, S., Ejaz, R. ., Husna, A. U., Azam, A., Laila Rubab, S. ., Ghulam Nabi, Ullah, S., & Akhter, S. . (2021). L-Carnitine Improves Cytoprotection during Cryopreservation: A case study on Nili-Ravi Buffalo Sperm. Abasyn Journal of Life Sciences, 4(Issue 1), 80-85. Retrieved from https://ajlifesciences.com/ojs/index.php/ajls/article/view/123

Issue

Vol. 4 No. Issue 1 (2021)

Section

Full Length Research Article

Copyright (c) 2021 Saima Qadeer, Rabea Ejaz, Asma Ul Husna, Asima Azam, Syeda Laila Rubab, Ghulam Nabi, Sana Ullah, Shamim Akhter

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