AJ Life Sci. 2021, 4 (1): 40-50

Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated from Hospital Environment

Munzer Ullah *1, Hayat Ullah2,Khaliq Noor 3 ,Maliha Sarfraz 4 ,Misbah Ullah Khan 5 ,Uzma Bibi 2 ,Ghulam Nabi 6 ,Maheen Kanwal 1,Kainat Ramzan 1and Ahmed M. Metwaly 7

1 Department of Biochemistry, University of Okara, Okara-56300, Punjab, Pakistan.
2 Department of Chemistry, University of Okara, Okara-56300, Punjab, Pakistan
3 Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
4 Department of Zoology, Faculty of Life Sciences, The Women University, Multan-66000, Pakistan
5 Center of Nano Science, University of Okara, Okara-56300, Punjab, Pakistan
6 6Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, college of Life Sciences, Hebei Normal University, Shijiazhuang, China
7 Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt


Pseudomonas aeruginosa is a leading cause of disease and death particularly in cystic fibrosis patients and also considered resistance to chemotherapeutic agents. Therefore, it is very difficult to remove the Pseudomonas aeruginosa from the hospital environment by using simple techniques. In the contemporary study, biofilm mediated mechanism of various antimicrobial responses were analyzed. For this purpose, different Pseudomonas aeruginosa clinical isolates were collected from Pakistan medical institute Islamabad (PIMS) hospital and were investigated for pellicle formation. Pseudomonas aeruginosa isolates were studied for different groups of antibiotics including imipenem, meropenem, ceftazidime, amikacin, tobramycin, gentamicin, piperacillin, cefoperazone, and cefotaxime. The goal was to check antimicrobial susceptibility of pseudomonas aeruginosa which shows resistant to tobramycin, imipenem, meropenem, amikacin, gentamicin, cefotaxime, piperacillin, ceftazidime, cefoperazone. Additionally, in this study, Pseudomonas aeruginosa strains were also investigated for pellicle formation. In conclusion, this research work wills highlights the useful mechanism of antibiotics resistance to Pseudomonas aeruginosa infections in clinical practice.

Keywords: Antibiotics, Pseudomonas aeruginosa, antibiotics, Biofilm, Peliclle.




April 09, 2021

Received Revised

July 26, 2021


August 8, 2021

Available Online

September 11, 2021

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How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University