AJ Life Sci. 2020, 3 (2): 193-199

Bio efficacy of Eruca sativa oil against Callosobruchus chinensis on chickpea

Agha Mushtaque Ahmed 1, Ali Zachi Abdul Qadeer 2, Jamal Uddin Hajano 3 ,Aqeel Alyousuf 2 ,Shahjahan Rajput 4 ,Imran Ali Rajput 5 ,Din Muhammad Soomro 1 and Rawat Khan Rind 1

1 Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam,Sindh, Pakistan.
2 Department of Plant Protection, College of Agriculture, University of Basrah, Basrah, Iraq
3 Department of Pathology, Faculty of Crop Protection Sindh Agriculture University, Tandojam, Sindh, Pakistan.
4 Department of Entomology, Shaheed Zulfiqar Ali Bhutto Agricultural College, Dokri, Sindh,Pakistan.
5 PARC- Arid Zone Research Institute, Umerkot, Sindh, Pakistan.


Pulse beetle, Callosobruchus chinensis L. responsible for destroying qualitative and quantitative losses to most stored grains. Once the damage done by the beetle, the grains lose their nutritional and germination capacity and become unfit for either consumption. Seed treatment still mainly relies on heavy synthetic chemicals which cause residual effect and threaten public health. However, the natural plant products containing insecticidal activity have recently displayed a great scope as they tend to come up with low mammalian toxicity, less effect on environmental and broad public adoption. Therefore, an experiment was conducted with variable doses of Eruca sativa L. seed oil (0.5 ml, 1 ml, and 1.5 ml) against C. chinensis. The data were recorded for corrected mortality, repellent effects, and seed protectant at different intervals (24, 48, 72 hrs and 1 week). We found the higher corrected mortality (32.65%) at 1.5ml after 24 hrs. Later, the mortality percentage decreased at different intervals but remained higher at maximum dose of E. sativa. However, the overall highest mortality of C. chinensis was 50.00% at 1.5 ml and the lowest 41.00% at 0.5 ml. Meanwhile, the index showed repellent effect with R.I value <1 in all treatments at different intervals. The seed protectant data showed that the minimum number of 1.66+-0.33 eggs laid on treated seeds at 1.5 ml and 8.00+-1.15 eggs on untreated seeds in choice experiment. In total, maximum number of 17.33+-4.93 eggs laid at 0.5 ml in choice experiment and minimum 4.83+-1.51 eggs at 1.5ml. However, in no choice experiment, the maximum number of 51.00+-4.58 eggs on control treatment and minimum 0.33+-0.33 eggs on 1.5 ml were laid by pulse beetle. The most effective dose of 1.5ml caused maximum mortality, repellent and seed protectant.

Keywords: Callosobruchus chinensis, Chickpea, Eruca sativa, Repellent, Mortality.


September 23, 2020

Received Revised

October 05, 2020


October 10, 2020

Available Online

December 31, 2020

Corresponding author email:


How to Cite

Ahmed MA, Qadeer AZA, Hajano JU, Alyousuf A, Rajput S, Rajput IA, Soomro DM, Rind RK. Bio efficacy of Eruca sativa oil against Callosobruchus chinensis on chickpea. Abasyn Journal of Life Sciences 2020; 3(2): 193-199.

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University