AJ Life Sci. 2020, 3 (1)

GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica

Faris Bakheet Alsaedi , Ihsan Ullah* MajedAl-Shaer, Rashad R. Al-Hindi and Khalid M.S. Al-Ghamd

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia



Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartiiand 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds

Keywords Azadirachta indica, antibacterial activities, Enterobacteriaceae, GC-MS, secondary metabolites, antibiotic resistant genes

Received

January 22, 2020

Received Revised

March 07, 2020

Accepted

March 08, 2020

Available Online

June 29, 2020


Corresponding author email:

iullah@kau.edu.sa

How to Cite

Alsaedi FB, Alzaidi SA, AlHindi RR, AL-Ghamdi KMS, Ullah I. Metabolic GC-MS Profiling of Anti-bacter metabolic compounds from the extract of Azadirachta indica Abasyn Journal of Life Sciences 2020; 3(1): 17- 23.

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University