Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartiiand 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds
Keywords Azadirachta indica, antibacterial activities, Enterobacteriaceae, GC-MS, secondary metabolites, antibiotic resistant genes
https://doi.org/10.34091/AJLS.3.1.3
ReceivedJanuary 22, 2020
Received RevisedMarch 07, 2020
AcceptedMarch 08, 2020
Available OnlineJune 29, 2020
iullah@kau.edu.sa
Alsaedi FB, Alzaidi SA, AlHindi RR, AL-Ghamdi KMS, Ullah I. Metabolic GC-MS Profiling of Anti-bacter metabolic compounds from the extract of Azadirachta indica Abasyn Journal of Life Sciences 2020; 3(1): 17- 23.