AJ Life Sci. 2020, 3 (1): 1-8

Immunochromatographic Technique for Seropositivity and PCR Based Molecular Diagnosis for Hepatis B and C Virus in a General Population of Mansehra Pakistan

Farzana Khan *1 , Zia Alam *1 Mubassir Shah, *1 Latif Ullah*1Hajra Shahana*2

1 Department of Biotechnology Abdul Wali Khan University Mardan KP, Pakistan
2 Department of Chemistry Women University Swabi Pakistan



Abstract

Hepatitis B and C infections are known to be the major public health issue around the world. Our focusis to investigate the predominance of HBV and HCV infections in the general population of district Mansehra, Khyber Pakhtunkhwa, Pakistan. Blood samples were taken from 652 individuals and were shifted to Alkhedmat laboratory Mansehra and Department of Microbiology Hazara University Mansehra. Serum were isolated from blood samples and screen it by ICT device Kit (Accurate Diagnostics, Houston, Texas, USA). The ICT positive samples were then refined by PCR method. Total 652 individuals [males (368), females (284)] were screened for HBV and HCV infections in which total 37 (5.67%) [males (6.52%) and females (4.57%)] were found positive for HBsAg and 42 (6.44%) [males (7.33%) and females (5.28%)] were found positive for Anti HCV respectively. The ratio of these viral infections is more predominant as compare to other viral infections in Mansehra, Pakistan. The rate of HBV and HCV prevalence is increasing day by day in these areas. Communities with more than 5% HBV and HCV disease, mass vaccination and awareness programs should be undertaken as a matter of urgency.

Keywords HBV, HCV, ICT, PCR, Risk factors, Mansehra

Received

December 29, 2019

Received Revised

March 21, 2020

Accepted

March 22, 2020

Available Online

June 29, 2020


Corresponding author email:

Farzana_khan@awkum.edu.pk

How to Cite

Khan F, Alam Z, Shah Mubassir, Ullah Latif, Shahana H. Immunochromatographic Technique for Seropositivity and PCR Based Molecular Diagnosis for Hepatis B and C Virus in a General Population of Mansehra Pakistan. Abasyn Journal of Life Sciences 2020; 3(1): 1-8..

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University