AJ Life Sci. 2019, 2 (2): 42-48

Hepatitis B Virus Infection in the Healthy Volunteers: A Screening Campaign in Nowshera Khyber Pakhtunkhwa, Pakistan

Zia Alam1, Mubassir Shah1 Mudassir Khan2, Kainat Hazrat1 , Waqar Ali1 , Farzana Khan1 , AzizAhmad3, Fazal Jalil1*

1Department of Biotechnology Abdul Wali Khan University Mardan, KPK, Pakistan.

2Department of Healthcare Biotechnology, Atta Ur Rahman School of Applied Biosciences (ASAB), NationalUniversity of Science and Technology (NUST) Islamabad, Pakistan.

3Medical Lab Technology Faculty of Peshawar, KPK, Pakistan


Hepatitis B viral infection (HBV) is a genuine worldwide general medical issue. The aim of this study was to find the epidemiology of HBV infection with common risk factors among the people of Nowshera KhyberPakhtunkhwa, Pakistan. A camp was conducted for HBV screening in Nowshera City (September 2018) in which 1180 volunteers participated. Blood (5ml) was taken from volunteers in medical camp and was transported to Aziz Biotech Medical Lab and Research Center Mardan,Pakistan. All the samples were initially screened for HBV surface antigen using ICT device kit (Accurate Diagnostics Canada). Positive samples were then subjected to Real time PCR to check active hepatitis B infectionamongst positive ICT samples. Out of 1180 volunteers 58 (4.91%) were found positive including 22 (4.82%) females and 36 (4.97%) males. The ICT positive samples were than refined by real-time PCR for active hepatitis Bvirus out of that 26 (44.82%) were found active by PCR which comprises 8 (36.36%) females and 18 (50%) males. The HBsAg ratio was greater in the Age-limit 21-30 years (5.67%) and 41-50 years (5.20%). The Sero-prevalenceof HBV infection is higher in Nowshera region. The prevalence ratio among males is greater than females and mostly infected females were married which shows that sexual interaction is the probable risk factor for HBV infection. The rural communities are illiterate and unaware of the causative agents, spreading and the consequences of HBV infection. Thus, toovercome the incidence of HBV infection, we must educate the ordinary citizens about Hepatitis B virus.

KeywordsHBV Infections, Nowshera, Pakistan, Risk Factors




August 2, 2019

Received Revised

August 28, 2019


August 28, 2019

Available Online

October 1, 2019

Corresponding author email:


How to Cite

Alam Z, Shah M, Khan M, Hazrat K, Ali W, Khan F, Ahmad A, Jalil F. Hepatitis B Virus Infection in the Healthy Volunteers: A Screening Campaign inNowshera Khyber Pakhtunkhwa, Pakistan. Abasyn Journal of Life Sciences 2019; 2(2): 42-48.

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University