AJ Life Sci. 2023, 7 (1)

Frequency of Different Microorganisms Isolated from the Diabetic Foot Ulcers and their Antibiotic Susceptibility Pattern in a tertiary care hospital Lahore: A Retrospective cohort study

Ariza Azam, Arooj Basharat,Roheen Shahid, Talha Mannan, Hina Awais, Fareeha Bashir

Department of Medical Lab Technology, Shalamar School of Allied Health Sciences, Lahore, Pakistan.

University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan.

The main prospective of this study to detect the frequency of different microorganisms isolated from Diabetic Foot Ulcer (DFUs) and also their antibiotic susceptibility pattern. On the basis of purposive sampling technique the medical record of 356 patients was collected who have Diabetic foot ulcer and visited the Sakina Begum Institute of Diabetes and Endocrine Research Lahore (SIDER) from May 2021 to May 2022. Only those samples were included in this study whose bacterial growth was identified through microbiological media and further antimicrobial sensitivity was confirmed by disc diffusion method. About 54.5% of diabetic foot ulcer patients were tested positive for infections by microbes; among which 62% samples had single organism growth and 37% had polymicrobial growth. 34% of the isolated microbes were gram positive while 66% were gram negative. Staphylococcus aureus was the most prevalent isolated organism because it showed the strong resistant pattern against cefotaxime, cefuroxime and ampicillin but vancomycin have the 58% sensitivity for gram positive bacteria. Most of the isolates showed resistance to common antibiotics, so appropriate antibiotic administration is necessary to reduce the resistance pattern in DFUs and needs to be timely treated to avoid from further infection.
Keywords: Diabetes, Foot ulcer, Infections, Micro-organisms, Sensitivity, Antibiotics




January 30, 2024

Received Revised

March 8, 2024


March 14, 2024

Available Online

March 14, 2024

Corresponding author email:


How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University