AJ Life Sci. 2022, 5 (2)

Comparative Analysis of Microbial Prevalence in Normal and Endometritic cows

Muhammad Amjad Khan1, Ammara Shakeel1, Asmara Imtiaz1, Humaira Niamat1*

1Department of Biology, Lahore Garrison University, Sector C, DHA Phase 6, Lahore, Pakistan


Endometritis, the inflammation of the endometrium, causes infertility in dairy herds. The aim of this study was to perform a comparative analysis of microbial prevalence in normal and endometritic cows. Therefore, cows were divided into four groups, comprising 10 cows in each. Group A: normal cows with no symptoms. Group B: Young stock (fit for breeding) cows suffering from endometritis. Group C: milking cows suffering from Endometritis. Group D: dry cows suffering from Endometritis. Three sterile swabs were collected from all cows of four groups, thus making a total of 120 samples. Bacterial isolation and identification were performed according to bergey's manual. Most common pathogens in the uterus of endometritic cows were Escherichia coli and then Trueperella pyogenes > Salmonella enterica > Klebsiella pneumonia > Bacillus subtilis > Fusobacterium necrophorum > Proteus vulgaris >Staphylococcus aureus > Streptococcus pneumoniae. In normal cows, mostlynumbers of isolates were gram positive. S. aureus followed by S. pneumoniae,B.subtilis, T. pyogenes, E. coli and K. pneumoniae. In conclusion, highernumbers of pathogenic bacteria were found in endometritic cows ascompared to normal cows. E. coli, being gram negative pathogen is the majorcause of infection in female upper genital track and infertility. Appropriatecombination of antibiotics and anti-inflammatory medicines along with herbaltreatments should be adopted. Maintenance of proper hygiene of dairy herdsis inevitable.

Keywords: Trueperella pyogenes; Salmonella enterica; Klebsiella pneumonia; Fusobacterium necrophorum; Endometritis.




October 11, 2022

Received Revised

December 23, 2022


December 24, 2022

Available Online

December 25, 2022

Corresponding author email:


How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University