AJ Life Sci. 2022, 5 (1)

Characterization and identification of plant growth promoting endophytic bacterial strain IU10

Ihsan Ullah *1 , Muhammad Ali 2 and Muhammad Ibrahim 3

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

2 Department of Biotechnology, Bacha Khan University Charsadda, Pakistan.

3 Department of Forestry, Bamboo Research Institute, Nanjing Forestry University, Nanjing 11210037 China.


Growing evidence has suggested that plant growth-promoting endophytic bacteria can regulate, which can be used as biofertilizers, bio-stimulants, and biocontrol agent. In the present study, Bacillus subtills IU10 was isolated from plants and was subjected to advanced chromatography and spectroscopic techniques for the extraction and isolation of indole-3-acetic acid (IAA). Up to 10 ?g/mL of IAA was quantified in the bacterial extract using the [2H2]-IAA internal standards in GC-MS analysis. PVK agar containing Ca3(PO4)2 was used to measure the ability of IU10 to solubilize phosphate. The clear zones formed by phosphate solubilization were measured to assess the phosphate solubilization potential. Azurol-S (CAS) medium was used for measuring siderophore production. The orange halo circles were measured to quantify the siderophore production. Endophytic IU10 inoculated plants showed significantly improvement root to shoot length, biomass and chlorophyll as compared to control. The data indicates that IU10 produce phytohormones, siderophore and immobilize nutrients could be used as biofertilizer.

Keywords: phytohormones, nutrient immobilization, growth promotion, endophytes.




August 11, 2021

Received Revised

September 2, 2021


December 21, 2021

Available Online

September 17, 2022

Corresponding author email:


How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University