AJ Life Sci. 2021, 4 (2): 59-65

Invitro antimicrobial efficiency of Lawsonia inermis L (Henna) extracts against Multidrug Resistant Microorganisms

Shehla Javaid *1,2 , Amer Jamil 1,* , Hina Awais 2 , Samiah Shahid 3 , Saira Khan 4 and Hafiz Muhammad Rehman 2

1 Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

2 University Institute of Medical Lab Technology, University of Lahore, Lahore-Pakistan.

3 Institute of Molecular Biology and Biochemistry, University of Lahore, Lahore-Pakistan

4 Gulab Devi educational complex Lahore-Pakistan


Antibiotics are becoming less effective as drug resistance spreads throughout the world, making it ever more difficult to treat the disease. The effect of biotic stress on antimicrobial activity of Lawsonia inermis L. (Henna) was studied as an alternate to antimicrobial agents against multidrug resistant microorganisms. The study was carried out in Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture Faisalabad. The plant seedlings were given biotic stress with Fusarium solani. The induced extracts were harvested at different time intervals and the antimicrobial activity was investigated by disc diffusion method. The induced plant extracts revealed highest susceptibility against S. aureus (30+-0.02) and Pasteurella multocida (30+-0.01) at 12 hpi. The least activity was shown against all the strains at 0 hpi. The highest antifungal activity was found against Ganoderma lucidum (55+-0.05) at 24 hpi. The results demonstrated a significant difference in the antimicrobial activities of all the strains with and without fungal stress (P<0.05). The fungal induced extracts of Lawsonia inermis L (Henna) with enhanced antimicrobial activity may have the potential of being alternative and cost- effective agents against antimicrobial resistance.

Keywords: Antimicrobial; In-vitro; Lawsonia inermis; Drug resistance; Antibiotics.




August 31, 2021

Received Revised

December 29, 2021


December 29, 2021

Available Online

December 31, 2021

Corresponding author email:

shehla.javaid@mlt.uol.edu.pk and amerjamil@yahoo.com

How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University