AJ Life Sci. 2021, 4 (2): 46-58

Assessing the genetic potential of mustard genotypes for water stress through PEG-6000 treatments

Saima Bano 1 , Abdul Wahid Baloch 1,* , Shah Nawaz Mari 1 , M. Ubaidullah Shirazi 2 , Ghulam Hussain Jatoi 3 , Naila Gandahi 1 , Sajid Hussain Rao 1 and Muharam Ali 4

1 Department of Plant Breeding & Genetics

2 Nuclear Institute of Agriculture, Tandojam, Pakistan

3 Department of Agriculture, Mir Chakar Khan University, Sibi, Balochistan-Pakistan

4 Department of Biotechnology, SAU, Tandojam, Pakistan


Water stress is a primary constraint to achieve the goal of sustainable crop production. Water stress severely affects the seed production and oil yield of mustard genotypes. To overcome this problem, the development of water stress resilient mustard cultivars with potential seed and oil yield is a sustainable solution. Therefore, in vitro screening of mustard genotypes through PEG-6000 treatments were carried out on ten mustard genotypes. Along with control, two PEG-6000 levels were set to impose osmotic stress, such as, 6% and 10% PEG-6000. Data analyses depicted significant differences among all genotypes and between PEG-6000 treatments for all the studied traits, such as, shoot and root length, shoot and root fresh weight, shoot and root dry weight, K+ content, Ca++ content and K+/Ca++ ratio, demonstrating the availability of genetic differences in mustard genotypes for future stress breeding. Under PEG-6000 (6% and 10%) treatments, the genotypes like AARI-Canola, Khanpur Raya, Dhoom-1, Super Raya, Galaxy and Coral-432 exhibited high performance for seedling traits and less reduction due to PEG-6000 treatments. Hence, these mustard genotypes tend to provide useful genetic potential for water stress breeding.

Keywords: Oilseed crops, genetic variations, drought stress, seed yield.




August 30, 2021

Received Revised

December 25, 2021


December 26, 2021

Available Online

December 31, 2021

Corresponding author email:


How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University