AJ Life Sci. 2020, 3 (2): 66-72

Catalase Activity in Response to Metals in Freshwater Fish Catla catla

Kousar Aziz 1, Khalid Abbas 1*, Tanveer Ahmed 1,2*, Sajid Abdullah 1, Huma Naz 3 Syed Qaswar Ali Shah 3 Shahbaz Ahmad 1

1 Aquaculture Biotechnology Lab, Department of Zoology, Wildlife and Fisheries, University of Agriculture 38040, Faisalabad, Pakistan.
2 Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan.
3 Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan


Catalase activity in response to binary metal mixture of Zn + Ni was studiedin gills, hepatic, renal and cardiac tissues of Catla catla. A total of 30 fishfingerlings were kept in two glass aquaria (15 in controlled condition and 15in metal mixture containing aquarium) for the period of two weeks. Afterthe completion of the trial period, fish were dissected for different organcollection. The extracted organs were homogenized in phosphate buffer (50mM; pH 7.0). The activity of catalase was determined by absorption at 240nm by using the standard methods. The inferences showed higher catalaseactivity in liver (223.33 +-1 UmL-1), kidney (163.33 +- 0.7 UmL-1), gills (123.33+-0.9 UmL-1) and cardiac (120 +-3 UmL-1) tissues of Zn + Ni treated fish incomparison to controlled fish liver (116.66 +-2 UmL-1), kidney (101.66 +-1UmL-1), gills (96.66 +-0.66 UmL-1) and cardiac tissues (70 +-0.33 UmL-1) in thisstudy. Statistically, significant differences at p ? 0.05 was observed forcatalase activity between Zn + Ni stressed and control fish groups. While, indifferent organs of both group of fishes the catalase activity order wasobserved as hepatic > renal > gills > heart. Findings of this study would behelpful in monitoring aquatic ecosystems using fish antioxidant systemwhich acts as a bio-indicator of metal contamination.

Keywords: Heavy metals, Antioxidants, Major carp, Aquatic pollution,Biomarkers.




October 9, 2020

Received Revised

November 28, 2020


November 28, 2020

Available Online

December 31, 2020

Corresponding author email:

dr.abbas@uaf.edu.pk; tanvirahmeduaf@gmail.com

How to Cite

Aziz K, Abbas K, Ahmed T, Abdullah S, Naz Huma, Shah SQAS, Ahmad S. Catalase Activity in Response to Metals on Freshwater Fish Catla catla. Abasyn Journal of Life Sciences 2020; 3(2): 66-72.

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University