AJ Life Sci. 2025, 8 (2)

Estimation of Heterotic Effect of Mustard Genotypes (Brassica Juncea L.) Under Water Stress Conditions

Saima Bano, Sajid Hussain Rao, Abdul Wahid Baloch, Hajira Imran, Rabab Akram, Muhammad Arsal, and Asadullah

1Oilseeds Research Institute, Agriculture Research Sindh, Tandojam.

2Department of Plant Breeding & Genetics, SAU, Tandojam

3Department of Research and Development, UPL Pakistan (Arysta Life Science) PVT Ltd



Abstract

The current investigation aims to develop the genetic capability of seed yield and oil content genotypes based on genetic analysis (heterosis) under water-deficient environments. In this manner, 6 mustard cultivars, which were highly water stress-tolerant, like (AARI-Canola, Khanpur Raya, Dhoom-1, Super Raya, Galaxy, and Coral-432) hybridized using a half diallel fashion 6 × 6 and produced 15 F1 progeny for further genetic analysis. The research was conducted in randomized complete block design having three replications and two treatments, during 2019-20 at SAU, Tandojam. T1 Well-watered at (stem growth, flowering, silique formation and maturity stages) and T2 Water stressed at (stem growth and flowering stages), respectively. Total 8 traits were investigated (plant height, branches plant-1, seed yield plant-1, seed index, SPAD chlorophyll, relative water content, oil content and protein content). Mostly studied traits revealed significantly differed (p<0.05) for all variances like (mean squares) of genotypes, treatments and genotype×treatment interaction as well as for heterotic effects over better parents under both conditions. The hybrids AARI-Canola×Super Raya, AARI-Canola×Galaxy, AARI-Canola×Coral-432, Khanpur Raya×Coral-432 and Super Raya×Coral-432 demonstrated superior parent heterotic effects for seed yield and its related traits under water deficit. Therefore, these crossing materials are helpful for heterotic breeding on a genetic and water stress basis and could be used for the development of desired genotypes and crosses in future breeding as well.

 Keywords: Mustard, water deficit, heterosis, yield traits, oil traits

DOI

https://doi.org/10.5281/zenodo.17597237

Received

September 12, 2025

Received Revised

November 11, 2025

Accepted

November 12, 2025

Available Online

November 13, 2025


Corresponding author email:

sajidrao100100@gmail.com

How to Cite


Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University