AJ Life Sci. 2023, 6 (2)

Bacteriological profile and antibiogram patterns of pyogenic bacteria isolated from pus specimens at a tertiary care hospital, Islamabad

Zainab Jamal , Maryam Faheem , Itrat Noor , Zukhra Abbasi , Ubaid Ahmed Abbasi , Bushra Uzair

Department of Biological Sciences, International Islamic University, Islamabad, Pakistan

Hamdard College of Medicine and Dentistry, Hamdard University, Main Campus, Madinat ul-Hikma, Karachi, Pakistan


Pyogenic wound infections are a significant source of morbidity, due to the development of different resistant strains. Assessing antibiotic sensitivity patterns for pyogenic bacterial isolates from pus samples is essential for the identification of suitable antibiotic treatments for patients. Antibiotic resistance among various bacteria develops and spreads due to the careless use of antibiotics for treating diseases. The present six-month study was conducted to establish the bacteriological profile and antibiogram patterns through the analysis of bacterial isolates obtained from various pus-infected patients in the Department of Microbiology at a tertiary care hospital, Islamabad. Total of 373 pus samples were collected and processed by standard microbiological techniques for the identification of bacterial isolates by culturing them on selective and differential media. According to Clinical and Laboratory Standard Institute (CLSI) guidelines, the antibacterial sensitivity profiling was performed by using the Kirby-Bauer method. The most prevalent bacterial isolate identified was Staphylococcus aureus, accounting for 46% of cases, followed by Pseudomonas aeruginosa (23%), E. coli (7%), Klebsiella pneumoniae (6%), Proteus mirabilis (3.5%), Enterococcus (2%), Providencia stuarti (1.8%), Acinetobacter baumannii (0.39%), and Stenotrophomonas maltophilia (0.38%). The results indicated that Grampositive bacteria were highly sensitive to antibiotics like linezolid, vancomycin, rifampicin, teicoplanin, and minocycline. In contrast, Gramnegative bacteria showed greater susceptibility to ciprofloxacin, tigecycline, amikacin, and levofloxacin. The study provides the foundation for evidence-based therapy to reduce the unnecessary use of antibiotics, thereby ensuring successful treatment for pyogenic infections and helps in preventing the emergence of drug-resistant strains.

Keywords: pyogenic infection, antimicrobial resistance, morbidity, antibiotic susceptibility pattern, Kirby Bauer method




June 12, 2023

Received Revised

July 11, 2023


November 14, 2023

Available Online

November 28, 2023

Corresponding author email:


How to Cite

Abasyn Journal of Life Sciences , ISSN (online): 2663-1040, Published by Abasyn University